The train was travelling between Laem Chabang and Hua Takhae railway stations when it hit the bus at the railway crossing.
Thailand train and bus colloide kill at least 20 people today .
Provincial Gov, Maitree Tritilanond earlier told reporters that so far about 29 people were injured, but the number was revised upwards to 44, all but 13 of them discharged from hospital by early afternoon.
State Rail Thailand governor Nirut Maneephan confirmed the death toll at the site of the crash to reporters.
According to eyewitness accounts, the bus driver had stopped at the railway crossing for a train but he reportedly did not notice the alarm for another train coming from the other direction.
The bus was travelling from Samut Prakan province to a Buddhist temple in Chachoengsao when the collision happened.
The bus was crossing a railway track when the train smashed into it in Thailand. The tour bus in Thailand killing at least 20 people and injuring 30 others, officials have said injured taken to hospital
The bus, with 65 passengers on board, was crossing a railway track at around 8am local time in Chacheongsao, 50 miles (80km) east of Bangkok, when the train hit it.
Bus was travelling from Samut Prakan province to a Buddhist temple in Chachoengsao,District chief officer Prathueng Yookassem told Thailand’s PBS TV: “It was raining, perhaps, the driver did not see the train.”